Discover Revvity’s No-Wash Immunoassays: Fast, Sensitive, and Powerful Solutions

Revvity brings innovation to scientific discovery with its portfolio of powerful, no-wash immunoassay technologies: AlphaLISA, AlphaLISA SureFire Ultra, and HTRF.

Unlike traditional ELISA and Western blot techniques, these platforms deliver sensitive and reproducible results in as little as three hours without the need for wash steps or large sample volumes:

Trusted by researchers across multiple therapeutic areas—from oncology and immunology, to neuroscience, metabolic disease, and more—Revvity’s immunoassays support a wide range of applications, such as biomarker detection, cytokine profiling, and detailed analysis of critical signaling pathways. Whether you’re working with cell lines, tissue lysates, or other complex biological samples, these assays offer the flexibility and performance required to move your research forward with confidence.

About AlphaLISA Technology:

AlphaLISA (Amplified Luminescent Proximity Homogeneous Assay) is a bead-based, no-wash homogeneous assay platform, enabling the detection of analytes and a dynamic range of biological interactions, including protein-protein, protein-peptide, and protein-small molecule interactions. This technology utilizes streptavidin-coated Alpha Donor beads and AlphaLISA Acceptor beads. Upon excitation, a singlet oxygen molecule is generated in the Donor bead that triggers a series of chemical reactions in the Acceptor bead, resulting in the emission of light at 615 nm, which is directly proportional to the amount of analyte or binding events within the sample. Alpha technology allows for the direct detection of molecules of interest in a homogeneous format, eliminating the need for time-consuming wash steps.

About AlphaLISA SureFire Ultra Technology:

AlphaLISA SureFire Ultra (ALSU) is a subset of AlphaLISA technology offered by Revvity. Similarly to AlphaLISA, Donor and Acceptor beads are used with this platform. In ALSU systems, Donor beads are coated with streptavidin to capture a biotinylated detection antibody, while Acceptor beads are coated with a proprietary CaptSure™ agent to immobilize the other detection antibody labeled with a CaptSure tag. In the presence of a target protein, the antibodies bring the Donor and Acceptor beads into proximity. Upon excitation at 680 nm, a photosensitizer within the Donor bead converts ambient oxygen into an excited singlet state, which diffuses up to 200 nm and produces a chemiluminescent reaction in the Acceptor bead, emitting light at 615 nm. If an Acceptor bead is not in proximity to a Donor bead, little to no signal is produced over the background.

About HTRF Technology:

 
HTRF (Homogeneous Time-Resolved Fluorescence) is a homogeneous, no-wash immunoassay platform used to detect and measure proteins and molecular interactions. The technology employs two antibodies labeled with distinct fluorophores that bind simultaneously to the same target antigen. Upon excitation of the donor fluorophore by a light source such as a laser or flash lamp, energy is transferred to the acceptor fluorophore via Fluorescence Resonance Energy Transfer (FRET). The acceptor then emits fluorescence at a specific wavelength, typically 665 nm. The magnitude of the FRET signal is proportional to the concentration of antigen-antibody complexes present in the sample, allowing for quantitative analysis without the need for wash steps.

Applications:

- AlphaLISA: This platform is perfect for measuring cytokines, biomarkers, and other targets in complex biological samples.

- AlphaLISA SureFire Ultra: Our most sensitive platform is ideal for detecting protein phosphorylation, post-translational modifications, and intracellular signaling cascades. ALSU is also well-suited for PROTAC and protein degradation studies.

- HTRF: Excels in studying molecular interactions, including protein-protein interactions, kinase activity, receptor-ligand binding, and epigenetic modifications, as well as biomarker and cytokine measurement.

By integrating AlphaLISA and HTRF technologies into your workflows, you can advance your research through precise, reliable data and streamlined processes across a broad range of therapeutic areas.

If you have any questions or want to discuss your next project with us, please do not hesitate to reach out.

Carl Apgar

Sr. Sales Specialist, Revvity

carl.apgar@revvity.com 

 Additional Information: 

- AlphaLISA Technology Overview: 

- Alpha Assays

- AlphaLISA SureFire Ultra

- HTRF Technology:

- HTRF

Written by Juliette Seremak, PhD

Commercial Technical Writer (Drug Discovery Reagents), Revvity